This system combines three devices and software for automated measurements of complete step-wise oxygen equilibration curves of respiratory pigments and recise pH sampling over a broad range of temperatures and gas mixtures. Determine P50 values and Hill coefficients in undiluted blood, hemolysate or purified pigments, e.g., myoglobin, hemoglobin, hemocyanin, hemerythrin, leghemoglobin etc. The PC software interface allows for fully automated measurements, data acquisition and analysis making it easy to run multiple samples with minimum effort and time-consumption.
Use this patented BOBS™ instrument for determining P50 values and Hill coefficients for oxygen binding in hemoglobin and other pigments. Samples as small as 1.5 µL are placed in a modified diffusion chamber, in which pigment saturation is continuously monitored over a wide range of wavelengths (190-885 nm) resulting in highly reproducible oxygen binding curves. Digital Peltier temperature controllers and a gas humidification system allows for measurements over a wide range of sample temperatures (5-45° C) while avoiding sample desiccation. User-friendly Windows software for fully automated data colleciton and analysis minimizes time-consumption and enable the user to run multiple samples per hour.
This GMS instrument comprises four mass flow controllers to deliver a highly accurate flow of O2 (0.01 – 100%), CO2 (0.03 – 6%) and N2 gasses. An intuitive software interface allows for standard and custom protocols making automated operation easy and flexible. Exceptional precision in oxygen level is achieved by dedicating two mass flow controllers to regulating low and high oxygen flows, respectively. The GMS requires very little bench space in the lab due to the compact design. It includes dedicated software for Windows and is fully compatible with the Blood Oxygen Binding System (BOBS™).
The pH-1 Micro instrument is a precise single channel, temperature compensated pH meter, for use with fiber optic pH micro sensors. It requires a PC for operation and is controlled via included software for Windows, which also saves and visualizes the measured values.
This fiber optic micro pH sensor is housed inside a 70 mm long syringe needle compatible with the sample holder for our BOBS™ instrument to allow real-time measurements of pH in micro liter samples.
The pH sensitive tip of the optical fiber is protected inside a stainless steel needle, but for measurement purposes the tip can be exposed by pressing a plunger. This design allows for easy penetration through skin, a rubber septum or any other harsh material as well as for secure transportation and insertion.
Glass chamber with mL measuring cell and water jacket, for determining oxygen consumption of small aquatic animals (e.g. fish larvae or tissue samples) or do blood gas analysis for determining total oxygen and carbon dioxide content of small blood samples, e.g. method by Tucker, 1963.