Use this patented BOBS™ instrument for determining P50 values and Hill coefficients for oxygen binding in hemoglobin and other pigments. Samples as small as 1.5 µL are placed in a modified diffusion chamber, in which pigment saturation is continuously monitored over a wide range of wavelengths (190-885 nm) resulting in highly reproducible oxygen binding curves. Digital Peltier temperature controllers and a gas humidification system allows for measurements over a wide range of sample temperatures from 10-45° C while avoiding sample desiccation. User-friendly Windows software for fully automated data collection and analysis minimizes time-consumption and enable the user to run multiple samples per hour.