This system measures individual respiration in real-time in tiny organisms like Zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc., based on a 24-channel optical fluorescence oxygen reading device. Individual organism are placed inside gas-tight 80-1,700 µl glass chambers on a 24-well microplate footprint for real-time oxygen consumption rate measurements. Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot that can be re-calibrated and sterilized for multiple trials. The microplate and reader is placed inside an incubator for temperature control, or use an optional flow-through water bath and refrigerated circulator. A soft silicone gasket with an optional line (e.g. parafilm) is placed on top of the microplate to seal the 24 chambers in one go.
Upgrade this system with up to nine add-on systems each including an extra reader and plate, i.e., for measuring respiration in 240 organisms simultaneously.