LoliTrack 5 is a fast and intuitive Windows software for 2D and 3D video tracking and behavior analysis of multiple and focal animals based on contrast or background subtraction, i.e., no markers are needed. This major upgraded version features many improvements and additions as well as a much faster performance on regular PCs.
LoliTrack 5 works with most cameras and video file formats and can track organisms of any shape or size in one or multiple arenas. The program includes a video recording and editing tool, graphs, heat maps, and can export video or image files with data overlay or Excel data files with user-defined output.
A unique feature is that LoliTrack 5 can track numerous animals inside a single arena – even if the objects have the same (or different) shape, color and size. It can handle occlusions (e.g. collisions or crossing paths), and applications where animals can hide or move out of view. Any tracking errors are easily identified and corrected, if required. The user can pick out individual animals for tracking and analysis rather than having to track all of them. User-defined time intervals can be omitted or included for tracking.
With two cameras (or files), this software offers 3D video tracking and behavior analysis in focal animals, and export 3D models of trajectories for further visualizing and analyzing in other software.
Other applications include tracking organisms in 24-, 48- or 96-well microtiter plates for fast screening of activity levels or locomotion (e.g. for toxicological or biomedical assays). User-defined treatments can be added to aid statistical analysis.
LoliTrack 5 can identify specific body points (tail, head, midline center, center of gravity) and track these for studying kinematics (tail beat frequency, body oscillations, etc.) or specific behaviors (direction of orientation or movement, turning rate etc.).
Any number of user-defined zones of interest can be applied, in order to score behavior in and out of these in an automated way.
A free demo software allows you to test if LoliTrack 5 will work for your application. Alternatively, we offer free analysis and advice if you send us a video clip as we want to make sure that software works for your application too!
For video tracking example files, click here. For LoliTrack 5 FAQ, click here.
- X,Y coordinates for front, back, center of midline, and center of gravity
- Distance moved
- Speed and Velocity
- Direction of movement
- Direction of orientation
- Bend (i.e., angle of body for oscillations like tail beat frequency etc.)
- Turning rate of movement or orientation
- Active/Inactive time and %
- Time spent in zones
- Inter-Individual Distance (IID)
All the parameters can be calculated for individual animals, for groups of animals (treatments) or for each user-defined zone (e.g. time spent).