BOBS™ instrument

Features

• Identify oxygen binding in hemoglobin or your pigments of interest!
• P₅₀ values and Hill coefficients compare to published values for mammalian/fish/invertebrate blood.
• Collect full spectrum (UV-VIS) sample absorbance and get more information than with any other method.
• Identify absorbance peaks that best define the oxygenation and deoxygenation events.
• Isosbestic point for reference.
• Patent # WO2015000453 A3 from The Alfred Wegener Institute. Licensed exclusively to Loligo^® Systems.
• Reference: Oellermann, Pörtner & Mark (2014). Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in blood microvolumes. J. Exp. Biol. (DOI:10.1242/jeb.092726)

Use this patented BOBS™ instrument for determining P₅₀ values and Hill coefficients for oxygen binding in hemoglobin and other pigments. Samples as small as 1.5 µL are placed in a modified diffusion chamber, in which pigment saturation is continuously monitored over a wide range of wavelengths (190-885 nm) resulting in highly reproducible oxygen binding curves. Digital Peltier temperature controllers and a gas humidification system allows for measurements over a wide range of sample temperatures from 10-45° C while avoiding sample desiccation. User-friendly Windows software for fully automated data collection and analysis minimizes time-consumption and enable the user to run multiple samples per hour.