Prof. Mikko Nikinmaa from University of Turku, Finland, and his collaborators, used the microplate respirometer to analyze whether exposure to water-soluble fraction (WSF) of crude oil can be associated with changes in interindividual variability in phenotype in Daphnia magna. The 80 µl well microplate allowed the researchers to measure whole animal oxygen consumption rate and found that the variability of oxygen consumption rate decreased in 48 h 30 % WSF-exposed animals as compared to 10 % WSF-exposed or control animals. Furthermore, they observed that the oxygen consumption rates in F₁ and F₂ generations of unexposed and 10 % WSF-exposed Daphnia had decreased from parental F₀ generation and were also lower than in offspring of 30 % WSF-exposed specimens.

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Water-soluble fraction of crude oil affects variability and has transgenerational effects in Daphnia magna
Mikko Nikinmaa, Emilie Suominen and Katja Anttila (2019)
Aquatic Toxicology

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