Post doc Natalie Pilakouta from University of Glasgow, and her collaborators (Holar College), used the shuttle box system and ShuttleSoft to look at thermal preferences in three-spined stickleback (Gasterosteus aculeatus). The researchers find that fish from both warm (from freshwater warmed by geothermal activity) and cold (from ambient-temperature lakes) habitats had an average preferred temperature of 13 °C, which is considerably lower than the temperatures experienced by fish in warm habitats for most of the year. The results therefore claim to challenge the assumption that temperature preferences in ectotherms will readily evolve as a response to climate change.

Full article:

Temperature preference does not evolve in sticklebacks despite multigenerational exposure to elevated temperatures
Natalie Pilakouta, Shaun S. Killen, Bjarni K. Kristjánsson, Skúli Skúlason, Jan Lindström, Neil B. Metcalfe and Kevin J. Parsons (2019)
bioRxiv

Link to article