Microplate system (Core)

Features

• Individual sensor spot calibration (2-points)
• Faster sampling rate (<5 sec)
• Randomize wells (control/treatment)
• Graph/analyse data based on treatments
• Calculate respiration rates in real-time
• Compensate for background respiration
• Normalize oxygen values
• Operate multiple readers from a single PC
• Save data, graphs and statistics to Excel file

MicroResp™ is user-friendly software for Windows that will automate microplate respirometry, analyze respiration data, and get the most out of your oxygen microplate reader.

It replaces old and current programs entirely as this one piece of software handles device communication, calibration, data acquisition, analysis, and statistics.

Hence, this software will save you a lot of time for experiments and post-analysis, and provide more accurate oxygen and MO₂ data with a higher time resolution.

Features

• Real-time oxygen measurements in 24 wells
• Non-invasive & non-destructive measurements
• Compact design for use in incubators or on shakers
• Optional extension for monitoring of up to 240 samples 
• 1- and 2-points sensor calibration by user
• Sensors cleaned with chlorine or ethanol for repeated measurements

Use this 24-channel optical fluorescence oxygen system for high through-put (closed) microplate respirometry, or for monitoring oxygen conditions in (open) disposable plastic well plates. Using this reader with our re-usable 24-well glass microplates, oxygen consumption rates of tiny organisms can be measured in real-time inside 80-1700 µl closed gas-tight wells fitted with non-invasive and re-usable oxygen sensor spots. Up to 9 extra, and less costly, readers can be daisy-chained to the reader and splitter box included in this system, e.g., for real-time measurements of oxygen in up to 240 wells from a single PC.

Please consider optional MicroResp™ software for enhanced performance of the reader and quality of oxygen data.

We have developed and manufactured this re-usable 24-well glass microtiter plate for high through-put respiration measurements in tiny aquatic organisms like zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers sealed with an impermeable PCR film for real-time oxygen consumption rate measurements. The glass material provides non-permeable surfaces, unlike ordinary well plates that are made of gas permeable plastics. Each well is fitted with an oxygen sensor spot allowing for non-invasive optical oxygen measurements when placing the microplate on a special reader. The glass microplate and sensor spots can be cleaned with chlorine, ethanol etc. between trials and re-calibrated and thus used again and again.

Features

• Adhesive clear polyester film
• Waterproof film – prevents spills and contamination
• Reduces sample evaporation

Self-sticking waterproof PCR film used for sealing our glass microplates for microplate respirometry.

Watch this tutorial on how to attach the PCR film.

This microplate guide fits snug over the microplate reader offering a quick and effortless way of holding the microplate in place so that the 24 oxygen sensor spots are centered directly over the 24 LED light sources for optimum signal strength. The guide is made for applications where an incubator is used for temperature control of the microplate respirometry system. This guide is not needed if using the flow-through water bath for temperature control.