Microplate system (Core)

(24 channels/80uL)

Systems Microplate System SETUP MICROPLATE MULTIPLE SETUP EXPANDED Microplate Multiple X3 Microplate Reader Set Microplate Data Setup Microplate Diagonal Cut 3D Zoom In Microplate Glass Plate Spots PCR Film On Microplate Microplate Reader Components MICROPLATE SYSTEM
Systems Microplate System
SETUP MICROPLATE MULTIPLE SETUP EXPANDED
Microplate Multiple X3
Microplate Reader Set
Microplate Data Setup
Microplate Diagonal Cut 3D Zoom In
Microplate Glass Plate Spots
PCR Film On Microplate
Microplate Reader Components
MICROPLATE SYSTEM

Features

  • Oxygen consumption rate measurements in 24-well microplates
  • Available well volumes: 80, 200, 500, 940, and 1700 μl
  • Non-invasive & non-destructive measurements
  • Re-usable glass microplates with sealed wells for closed respirometry
  • Optical fluorescence sensors that can be re-calibrated for multiple trials
  • Compact design for use in incubators or on shakers
  • Real-time O2 measurements inside each gas-tight well
  • User-friendly Win 11 software for calibration, logging, statistics, and analysis
  • Connect up to 10 plate readers to a single PC

 

This simple high-throughput system measures individual respiration in tiny organisms like zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc., based on a 24-channel optical fluorescence oxygen reading device. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers sealed with an oxygen impermeable PCR film for real-time oxygen consumption rate measurements. The glass material provides non-permeable surfaces, unlike ordinary well plates that are made of gas permeable plastics. Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot for dynamic fluorescence quenching oxygen sensing. The sensors can be re-calibrated and sterilized for multiple trials. The entire setup can be placed inside an incubator for temperature control, or use our optional flow-through water bath and a circulating bath. A transparent self-adhesive sealing film is applied on top of the microplate to seal all 24 chambers in one go.

Higher throughput?

Upgrade this system with up to nine add-on systems each including an extra reader and plate, i.e., for measuring respiration in 240 organisms simultaneously.

Data

This is product data for the selected system:

Included

These products are included in the system:

Software

1
MicroResp™ v1 automated microplate respirometry software
MICRORESP PRODUCT (1)

Features

  • Individual sensor spot calibration (2-points)
  • Faster sampling rate (<5 sec)
  • Randomize wells (control/treatment)
  • Graph/analyse data based on treatments
  • Calculate respiration rates in real-time
  • Compensate for background respiration
  • Normalize oxygen values
  • Operate multiple readers from a single PC
  • Save data, graphs and statistics to Excel file

 

MicroResp™ is user-friendly software for Windows that will automate microplate respirometry, analyze respiration data, and get the most out of your oxygen microplate reader.

It replaces old and current programs entirely as this one piece of software handles device communication, calibration, data acquisition, analysis, and statistics.

Hence, this software will save you a lot of time for experiments and post-analysis, and provide more accurate oxygen and MO2 data with a higher time resolution.

Instrument

1
Microplate reader set (24 channels)
Microplate Reader Set

Features

  • Real-time oxygen measurements in 24 wells
  • Non-invasive & non-destructive measurements
  • Compact design for use in incubators or on shakers
  • Optional extension for monitoring of up to 240 samples 
  • 1- and 2-points sensor calibration by user
  • Sensors cleaned with chlorine or ethanol for repeated measurements

 

Use this 24-channel optical fluorescence oxygen system for high through-put (closed) microplate respirometry, or for monitoring oxygen conditions in (open) disposable plastic well plates. Using this reader with our re-usable 24-well glass microplates, oxygen consumption rates of tiny organisms can be measured in real-time inside 80-1700 µl closed gas-tight wells fitted with non-invasive and re-usable oxygen sensor spots. Up to 9 extra, and less costly, readers can be daisy-chained to the reader and splitter box included in this system, e.g., for real-time measurements of oxygen in up to 240 wells from a single PC.

Please consider optional MicroResp™ software for enhanced performance of the reader and quality of oxygen data.

Accessories

1
24-well glass microplate (80 uL)
GLASS PLATE 80 SPOTS

We have developed and manufactured this re-usable 24-well glass microtiter plate for high through-put respiration measurements in tiny aquatic organisms like zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers sealed with an impermeable PCR film for real-time oxygen consumption rate measurements. The glass material provides non-permeable surfaces, unlike ordinary well plates that are made of gas permeable plastics. Each well is fitted with an oxygen sensor spot allowing for non-invasive optical oxygen measurements when placing the microplate on a special reader. The glass microplate and sensor spots can be cleaned with chlorine, ethanol etc. between trials and re-calibrated and thus used again and again.

1
Microplate sealing film (100 pcs)
PCR Film On Microplate

Features

  • Adhesive clear polyester film
  • Waterproof film – prevents spills and contamination
  • Reduces sample evaporation

 

Self-sticking waterproof PCR film used for sealing our glass microplates for microplate respirometry.

Watch this tutorial on how to attach the PCR film.

1
Guide for microplate
Guide For Microplate

This microplate guide fits snug over the microplate reader offering a quick and effortless way of holding the microplate in place so that the 24 oxygen sensor spots are centered directly over the 24 LED light sources for optimum signal strength. The guide is made for applications where an incubator is used for temperature control of the microplate respirometry system. This guide is not needed if using the flow-through water bath for temperature control.

Optional

These products are optional for the selected system:

Systems

Systems Microplate System (Add On)
From 5,145.00 (EUR)
Microplate system (Add-on)

(24 channels/80uL)

Accessories

Water Bath For Microplate
Water bath for microplate

Bio Shaker Empty
Multi Bio 3D

Programmable shaker

PLATE ROLLER ISO
Plate roller for microplate sealing film

Compression Block
Compression block for microplate

Silicone Pad
Silicone pad

Files

These files can help you with the selected system:

Microplate

Title
Description
Version
Get
Title
MicroResp™
Description
A step by step guide on how to set up and use the software
Version
1.6
Get
Title
Microplate reader
Description
A data sheet for the microplate reader
Version
1.0.1
Get
Title
MicroResp™ - Keylok
Description
MicroResp™ software for Windows 11/10
Version
1.1.0
Get