Features

• Oxygen consumption rate measurements in 24-well microplates
• Available well volumes: 80, 200, 500, 940, and 1700 μl
• Non-invasive & non-destructive measurements
• Re-usable glass microplates with sealed wells for closed respirometry
• Optical fluorescence sensors that can be re-calibrated for multiple trials
• Compact design for use in incubators or on shakers
• Real-time O₂ measurements inside each gas-tight well
• User-friendly Win 11 software for calibration, logging, statistics, and analysis
• Connect up to 10 plate readers to a single PC

This simple high-throughput system measures individual respiration in tiny organisms like zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc., based on a 24-channel optical fluorescence oxygen reading device. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers sealed with an oxygen impermeable PCR film for real-time oxygen consumption rate measurements. The glass material provides non-permeable surfaces, unlike ordinary well plates that are made of gas permeable plastics. Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot for dynamic fluorescence quenching oxygen sensing. The sensors can be re-calibrated and sterilized for multiple trials. The entire setup can be placed inside an incubator for temperature control, or use our optional flow-through water bath and a circulating bath. A transparent self-adhesive sealing film is applied on top of the microplate to seal all 24 chambers in one go.

Higher throughput?

Upgrade this system with up to nine add-on systems each including an extra reader and plate, i.e., for measuring respiration in 240 organisms simultaneously.

Features

• Oxygen consumption rate measurements in 24-well microplates
• Available well volumes: 80, 200, 500, 940, and 1700 μl
• Non-invasive & non-destructive measurements
• Re-usable glass microplates with sealed wells for closed respirometry
• Optical fluorescence sensors that can be re-calibrated for multiple trials
• Compact design for use in incubators or on shakers
• Real-time O₂ measurements inside each gas-tight well
• User-friendly Win 11 software for calibration, logging, statistics, and analysis
• Connect up to 10 plate readers to a single PC

This add-on system will upgrade your (core) microplate system with an additional 24 channels. It includes everything needed for microplate respiration measurements including an extra reader that is simply daisy-chained to the first reader. Add up to nine extra readers in this way for simultaneous measurements in up to 240 chambers/wells from a single PC.