FAQ

To clean the glass microplate, use ethanol (<70 % v/v), bleach (<3% H₂O₂), or mild detergent, and rinse with demi water. Then dry. If using ethanol to sterilize the spots/wells, then dry the plate thoroughly, at least 2 days at 50-60 °C in an oven, to ensure that all residues have left the sensor dye matrix.

It is recommended to perform a manual calibration after cleaning/sterilizing.

Step-by-Step Instructions using bleach

1. Fill each well with 2-2.5% bleach, and ensure that there are no air bubbles in the wells.
2. Leave for 10-20 Minutes.
3. Empty the wells, and rinse each well thoroughly with demi water.
4. Let the wells dry overnight in the dark.
5. Perform a manual calibration before starting a new experiment.

To get oxygen consumption rates per volume  rather than per well (or organism) you turn off the “Organisms per well” in the settings section of the Experiment tab, as shown in the picture below, the software will then calculate the MO₂ based on the Well volume you set in the same section.

To select the preferred MO₂ unit, go to the Settings tab in the Analysis section, which is shown in the image below, here you find the new MO₂ units per volume by clicking on the dropdown menu and selecting your preferred unit.

The pop-up with the error message "No splitter found - please connect splitter with SDR" typically comes from a missing driver.

For most PCs, this driver is distributed alongside Windows updates, but in certain cases it is missing. When the driver is missing, the USB splitter cable is not recognized by Windows resulting in the error message.

To fix it, please do the following steps:

1. Download the driver from this link
   
   
2. Unzip and install the driver
   
   
3. Restart your PC
   
   
4. Try to connect again via MicroResp